Chimeric feline coronaviruses that encode type II spike protein on type I genetic background display accelerated viral growth and altered receptor usage.
Identifieur interne : 002569 ( Main/Exploration ); précédent : 002568; suivant : 002570Chimeric feline coronaviruses that encode type II spike protein on type I genetic background display accelerated viral growth and altered receptor usage.
Auteurs : Gergely Tekes [Allemagne] ; Regina Hofmann-Lehmann ; Barbara Bank-Wolf ; Reinhard Maier ; Heinz-Jürgen Thiel ; Volker ThielSource :
- Journal of virology [ 1098-5514 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Chats, Chimérisme, Coronavirus félin (croissance et développement), Coronavirus félin (génétique), Coronavirus félin (physiologie), Cricetinae, Cytométrie en flux, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Gènes viraux, Lignée cellulaire, Protéines de l'enveloppe virale (génétique), Récepteurs viraux (physiologie).
- MESH :
- croissance et développement : Coronavirus félin.
- génétique : Coronavirus félin, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- physiologie : Coronavirus félin, Récepteurs viraux.
- Animaux, Chats, Chimérisme, Cricetinae, Cytométrie en flux, Glycoprotéine de spicule des coronavirus, Gènes viraux, Lignée cellulaire.
English descriptors
- KwdEn :
- Animals, Cats, Cell Line, Chimerism, Coronavirus, Feline (genetics), Coronavirus, Feline (growth & development), Coronavirus, Feline (physiology), Cricetinae, Flow Cytometry, Genes, Viral, Membrane Glycoproteins (genetics), Receptors, Virus (physiology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Coronavirus, Feline.
- growth & development : Coronavirus, Feline.
- physiology : Coronavirus, Feline, Receptors, Virus.
- Animals, Cats, Cell Line, Chimerism, Cricetinae, Flow Cytometry, Genes, Viral, Spike Glycoprotein, Coronavirus.
Abstract
Persistent infection of domestic cats with feline coronaviruses (FCoVs) can lead to a highly lethal, immunopathological disease termed feline infectious peritonitis (FIP). Interestingly, there are two serotypes, type I and type II FCoVs, that can cause both persistent infection and FIP, even though their main determinant of host cell tropism, the spike (S) protein, is of different phylogeny and displays limited sequence identity. In cell culture, however, there are apparent differences. Type II FCoVs can be propagated to high titers by employing feline aminopeptidase N (fAPN) as a cellular receptor, whereas the propagation of type I FCoVs is usually difficult, and the involvement of fAPN as a receptor is controversial. In this study we have analyzed the phenotypes of recombinant FCoVs that are based on the genetic background of type I FCoV strain Black but encode the type II FCoV strain 79-1146 S protein. Our data demonstrate that recombinant FCoVs expressing a type II FCoV S protein acquire the ability to efficiently use fAPN for host cell entry and corroborate the notion that type I FCoVs use another main host cell receptor. We also observed that recombinant FCoVs display a large-plaque phenotype and, unexpectedly, accelerated growth kinetics indistinguishable from that of type II FCoV strain 79-1146. Thus, the main phenotypic differences for type I and type II FCoVs in cell culture, namely, the growth kinetics and the efficient usage of fAPN as a cellular receptor, can be attributed solely to the FCoV S protein.
DOI: 10.1128/JVI.01568-09
PubMed: 19906918
Affiliations:
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Le document en format XML
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xml:lang="en">Persistent infection of domestic cats with feline coronaviruses (FCoVs) can lead to a highly lethal, immunopathological disease termed feline infectious peritonitis (FIP). Interestingly, there are two serotypes, type I and type II FCoVs, that can cause both persistent infection and FIP, even though their main determinant of host cell tropism, the spike (S) protein, is of different phylogeny and displays limited sequence identity. In cell culture, however, there are apparent differences. Type II FCoVs can be propagated to high titers by employing feline aminopeptidase N (fAPN) as a cellular receptor, whereas the propagation of type I FCoVs is usually difficult, and the involvement of fAPN as a receptor is controversial. In this study we have analyzed the phenotypes of recombinant FCoVs that are based on the genetic background of type I FCoV strain Black but encode the type II FCoV strain 79-1146 S protein. Our data demonstrate that recombinant FCoVs expressing a type II FCoV S protein acquire the ability to efficiently use fAPN for host cell entry and corroborate the notion that type I FCoVs use another main host cell receptor. We also observed that recombinant FCoVs display a large-plaque phenotype and, unexpectedly, accelerated growth kinetics indistinguishable from that of type II FCoV strain 79-1146. Thus, the main phenotypic differences for type I and type II FCoVs in cell culture, namely, the growth kinetics and the efficient usage of fAPN as a cellular receptor, can be attributed solely to the FCoV S protein.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Bank Wolf, Barbara" sort="Bank Wolf, Barbara" uniqKey="Bank Wolf B" first="Barbara" last="Bank-Wolf">Barbara Bank-Wolf</name><name sortKey="Hofmann Lehmann, Regina" sort="Hofmann Lehmann, Regina" uniqKey="Hofmann Lehmann R" first="Regina" last="Hofmann-Lehmann">Regina Hofmann-Lehmann</name><name sortKey="Maier, Reinhard" sort="Maier, Reinhard" uniqKey="Maier R" first="Reinhard" last="Maier">Reinhard Maier</name><name sortKey="Thiel, Heinz Jurgen" sort="Thiel, Heinz Jurgen" uniqKey="Thiel H" first="Heinz-Jürgen" last="Thiel">Heinz-Jürgen Thiel</name><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Tekes, Gergely" sort="Tekes, Gergely" uniqKey="Tekes G" first="Gergely" last="Tekes">Gergely Tekes</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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